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KMID : 0358920200470020120
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Journal of the Korean Academy of Pedodontics
2020 Volume.47 No. 2 p.120 ~ p.127
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Antimicrobial Effect of Photodynamic Therapy Using Plaque Disclosing Agent
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Kho Jun-Hee
Park Ho-Won
Lee Ju-Hyun
Seo Hyun-Woo
Lee Si-Young
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Abstract
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The aim of this study was to evaluate the antimicrobial effect of photodynamic therapy (PDT) using plaque disclosing agent, 10 - 20 mM erythrosine, as a photosensitizer. Multispecies cariogenic biofilms containing Streptococcus mutans , Lactobacillus casei and Candida albicans were formed on hydroxyapatite disc. 20 μM, 10 mM and 20 mM erythrosine were applied as a photosensitizer for 3 minutes, and then light-emitting diode (LED) irradiated for 24 seconds. Colony-forming unit (CFU) were measured and biofilms were observed using confocal laser scanning microscopy (CLSM). CFU were significantly decreased in the PDT groups using 10 - 20 mM erythrosine (10 mM, 20mM) and the results were also confirmed by CLSM. This study confirms the high antimicrobial effect of photodynamic therapy using plaque disclosing agent as a photosensitizer.
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KEYWORD
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Photodynamic therapy, Erythrosine, Plaque disclosing agent, Multispecies biofilm
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